soleus sol muscles  (Dawley Inc)

Journal: The FASEB Journal

Article Title: Methyltransferase-like 21e inhibits 26S proteasome activity to facilitate hypertrophy of type IIb myofibers

doi: 10.1096/fj.201900582R

Figure Lengend Snippet: The expression of Mettl21e is associated with Mstn KO in fast muscles. A) Mettl21e expression in GA muscles from WT and postdevelopmental Mstn KO mice. B) Mettl21e expression in TA muscles from WT and constitutive Mstn KO mice. C) Mettl21e expression in differentiated myoblasts treated with follistatin. D) Mettl21e expression in myoblasts with Mstn overexpression. E) Mettl21e expression in GA muscles with anti-Mstn antibody JA16 or PBS. F) Immunostaining of type IIb (Myh4, Red) and type IIa (Myh2, Green) myofibers in EDL muscles of WT and Mstn KO mice. G, H) qPCR analysis of Myh genes (G) and Mettl21e (H) in EDL muscles of WT and Mstn KO mice. Scale bars, 100 µm. I) Immunostaining of type I (Myh7, Red) and type IIa (Myh2, Green) myofibers in SOL muscles of WT and Mstn KO mice. Scale bars, 100 µm. J, K) qPCR analysis of Myh genes (J) and Mettl21e (K) in SOL muscles of WT and Mstn KO mice. The values in A and E were originated from the published microarray data. Error bars represent mean + sd of 6 mice (B, G, H, J, K) or 5 independent biologic experiments (C, D). *P < 0.05 (Student’s t test).

Article Snippet: Single myofiber isolation Extensor digitorum longus (EDL) and soleus (SOL) muscles were removed carefully and digested with 2 mg/ml collagenase type 1 (CLS-1; Worthington Biochemical, Lakewood, NJ, USA) in DMEM (MilliporeSigma) for 45 min at 37°C.

Techniques: Expressing, Over Expression, Immunostaining, Microarray

Journal: The FASEB Journal

Article Title: Methyltransferase-like 21e inhibits 26S proteasome activity to facilitate hypertrophy of type IIb myofibers

doi: 10.1096/fj.201900582R

Figure Lengend Snippet: The expression Mettl21e in different types of myofibers. A) qPCR showing relative mRNA levels of Mettl21c and Mettl21e in different tissues. B) qPCR analysis of Mettl21c and Mettl21e in fast-twitch (TA, EDL, and GA) and slow-twitch (SOL) muscles; n = 6 independent biologic experiments with 3 technical repeats. C) qPCR analysis of Myh genes in isolated myofibers from SOL and EDL muscles of 4 mice. Individual myofibers were grouped into type I (blue), IIa (red), IIx (green), and IIb (gray). D) qPCR analysis of Mettl21c and Mettl21e in individual myofibers. Inset: the average expression of Mettl21c and Mettl21e in different types of myofibers. Error bars represent mean + sd *P < 0.05 (1-way ANOVA).

Article Snippet: Single myofiber isolation Extensor digitorum longus (EDL) and soleus (SOL) muscles were removed carefully and digested with 2 mg/ml collagenase type 1 (CLS-1; Worthington Biochemical, Lakewood, NJ, USA) in DMEM (MilliporeSigma) for 45 min at 37°C.

Techniques: Expressing, Isolation

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: Hematoxylin and eosin staining of rat skeletal muscles. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm.

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: (A–D) Representative Picrosirius Red staining images. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm. (E) Quantification of Picrosirius Red-positive collagen fiber content (% area) in the EDL and SOL of each age group. Data are presented as mean ± standard error of the mean (n=6 per group). *p<0.01 between groups (one-way analysis of variance with Bonferroni post hoc test).

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: (A–D) Representative hematoxylin staining images. Left and right images represent the extensor digitorum longus (EDL) and soleus (SOL) muscles, respectively. Panels A and B show the young group, whereas panels C and D show the middle-aged group. Scale bars, 50 μm. (E) Analysis of the number of nuclei per unit area (mm 2 ) in the EDL and SOL of each age group. Data are presented as mean ± standard error of the mean (n=6 per group). *p<0.01 between groups (one-way analysis of variance with Bonferroni post hoc test).

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Staining, Muscles

Journal: Journal of Toxicologic Pathology

Article Title: Intramuscular collagen accumulation in different types of skeletal muscle fibers in middle-aged male rats

doi: 10.1293/tox.2025-0072

Figure Lengend Snippet: Spearman’s rank correlation coefficients showing the relationship between collagen fiber content (Sirius red % area) and the number of nuclei per unit area (mm 2 ) in the extensor digitorum longus (EDL) and soleus (SOL) muscles of all animals (n=6 matched data points for each age group and each muscle). Open and black circles represent the EDL of the young and middle-aged groups, respectively. Open and black triangles represent the SOL of the young and middle-aged groups, respectively.

Article Snippet: The extensor digitorum longus (EDL) and soleus (SOL) muscles from male Sprague–Dawley (SD) rats were collected and sectioned.

Techniques: Muscles